pPB-CAG-Venus-Akaluc
(Plasmid
#209768)
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PurposeVenus-Akaluc is inserted into pPB-PGK-destination (Plasmid #60436).
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Depositing Lab
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Depositing OrganizationRIKEN BioResource Research Center
Located in Japan -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 209768 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 209768-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 209768-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepPB-PGK-destination
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Backbone manufacturerAustin Smith
- Backbone size w/o insert (bp) 7725
- Total vector size (bp) 10101
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Vector typeMammalian Expression, Luciferase ; PiggyBac Transposon
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVenus-Akaluc
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SpeciesSynthetic
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Insert Size (bp)2376
- Promoter CAG
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pCAGGS-5
- 3′ sequencing primer BGH-rev
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAkaluc is cloned from pcDNA3 Venus-Akaluc. The pcDNA3 Venus-Akaluc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15781).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
DNA (Catalog # 209768-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 209768-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-CAG-Venus-Akaluc was a gift from Yohei Hayashi (Addgene plasmid # 209768 ; http://n2t.net/addgene:209768 ; RRID:Addgene_209768)
