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Addgene

rUBR5_2383-2442_pGEX6P1
(Plasmid #209791)

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Item Catalog # Description Quantity Price (USD)
Plasmid 209791 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-6P-1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4984
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mlle domain of UBR5
  • Alt name
    PABC domain of UBR5
  • Species
    R. norvegicus (rat)
  • Mutation
    aa 2383-2442 only
  • Entrez Gene
    Ubr5 (a.k.a. Dd5, Rat100)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pGEX Fwd
  • 3′ sequencing primer pGEX Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rUBR5_2383-2442_pGEX6P1 was a gift from Kalle Gehring (Addgene plasmid # 209791 ; http://n2t.net/addgene:209791 ; RRID:Addgene_209791)

  • For your References section:

    Comparative peptide binding studies of the PABC domains from the ubiquitin-protein isopeptide ligase HYD and poly(A)-binding protein. Implications for HYD function. Lim NS, Kozlov G, Chang TC, Groover O, Siddiqui N, Volpon L, De Crescenzo G, Shyu AB, Gehring K. J Biol Chem. 2006 May 19;281(20):14376-82. doi: 10.1074/jbc.M600307200. Epub 2006 Mar 22. 10.1074/jbc.M600307200 PubMed 16554297
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