pFUSE-rabbitFc1-IL2ss-VHH(B12)-His-Myc
(Plasmid #209837)

• Purpose: Plasmid coding for a nanobody against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1
• Depositing Lab: Tim Brown
• Publication: pFUSE-RabbitFc1-IL2ss-B12-VHH(FGFR3)-His-Myc (unpublished)

Backbone

• Vector backbone: pFUSE
• Backbone manufacturer: Invivogen
• Backbone size w/o insert (bp): 3380
• Total vector size (bp): 4717
• Modifications to backbone: human beta (hb) globin polyadenylation (PA) signal
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: IL2-ss
• Alt name: Interleukin-2
• Alt name: pFuse-IL2ss
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 60
• Promoter: hEF1-HTLV promoter

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI, EcoRV, (unknown if destroyed)
• 3′ cloning site: BmtI, NheI (unknown if destroyed)
• 5′ sequencing primer: CGTTTTCTGTTCTGCGCC

Gene/Insert 2

• Gene/Insert name: VHH (B12 variant)
• Species: M. musculus (mouse)
• Insert Size (bp): 372
• Tags / Fusion Proteins:
  • x6 HisTag (C terminal on insert)
  • x3 MycTag (C terminal on insert)

Gene/Insert 3

• Gene/Insert name: Rabbit Fc Gamma 1
• Alt name: Rabbit IgG Fc
• Species: O. cuniculus (rabbit)
• Insert Size (bp): 687

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The VHH section (termed B12) was designed through phage display by the company Hybrigenics Services (Évry-Courcouronnes, France). The nanobody was seen to be specific towards FGFR3 (mouse) and not FGFR1 or FGFR2.