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pA-CBh.TP-CNOT8-V5H6.MCh.Puro
(Plasmid #209947)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209947 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 209947-D50 50 μg of DNA in Tris buffer $495
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DNA 209947-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pA
  • Total vector size (bp) 6721
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    CCR4-NOT transcription complex subunit 8
  • Alt name
    CNOT8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    881
  • GenBank ID
  • Entrez Gene
    CNOT8 (a.k.a. CAF1, CALIF, Caf1b, POP2, hCAF1)
  • Promoter CBh
  • Tags / Fusion Proteins
    • TP (MS2 coat protein) (N terminal on insert)
    • V5 epitope with H6 tag (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    mCherry fluorescent protein fused to puromycin resistance marker
  • Promoter CMV

Cloning Information for Gene/Insert 2

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Vector builder and GeneWiz

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pA-CBh.TP-CNOT8-V5H6.MCh.Puro was a gift from Kevin Weeks (Addgene plasmid # 209947 ; http://n2t.net/addgene:209947 ; RRID:Addgene_209947)

  • For your References section:

    Functional Proximity across an mRNA. Hatfield BM, Weidmann CA, McCutchin CA, Irving PS, Nelson IM, Felder S, Weeks KM. Biochemistry. 2025 Sep 16;64(18):3854-3865. doi: 10.1021/acs.biochem.5c00340. Epub 2025 Aug 12. 10.1021/acs.biochem.5c00340 PubMed 40792674
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