pBES1-LacOx50-mCh-bla
(Plasmid
#210260)
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PurposeIntegrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.
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Depositing Lab
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Depositing OrganizationImperial College London
Located in United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 210260 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 210260-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 210260-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGEM-3
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2257
- Total vector size (bp) 7491
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert name50xLacO repeats derived from: PMID: 12864855.
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SpeciesSynthetic; E. coli
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Insert Size (bp)1887
Gene/Insert 2
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Gene/Insert namemCherry
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Insert Size (bp)708
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Digest with AvrII + KpnI for transfection into trypanosomes.
DNA (Catalog # 210260-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 210260-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBES1-LacOx50-mCh-bla was a gift from Calvin Tiengwe (Addgene plasmid # 210260 ; http://n2t.net/addgene:210260 ; RRID:Addgene_210260) -
For your References section:
Imaging of genomic loci in Trypanosoma brucei using an optimised LacO-LacI system. Budzak J, Goodwin I, Tiengwe C, Rudenko G. Mol Biochem Parasitol. 2023 Dec;256:111598. doi: 10.1016/j.molbiopara.2023.111598. Epub 2023 Nov 3. 10.1016/j.molbiopara.2023.111598 PubMed 37923299
Map uploaded by the depositor.
