pMXS-mitoGshF-blasticidin
(Plasmid #210314)

• Purpose: Expresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cells
• Depositing Lab: Kivanc Birsoy
• Publication: Wang et al Nature. 2021 Nov;599(7883):136-140. doi: 10.1038/s41586-021-04025-w. Epub 2021 Oct 27.

Backbone

• Vector backbone: pMXS-IRES-Blast
• Backbone manufacturer: Cell Biolabs
• Backbone size w/o insert (bp): 5600
• Total vector size (bp): 2466
• Vector type: Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF
• Alt name: GshF
• Alt name: GshAB
• Species: Streptococcus thermophilus
• Insert Size (bp): 3913
• Mutation: Codon optimized for expression in human cells
• GenBank ID: CP000419, AAGS01000000, AAGS01000001-AAGS01000098, NZ_LR822015.1
• Entrez Gene: gshAB (a.k.a. H0506_RS06410, STHERMO_1423)
• Tags / Fusion Proteins:
  • FLAG peptide (N terminal on insert)
  • Lactobacillus brevis COXIV mitochondrial targeting sequence (N terminal on insert)
  • Homo sapiens ACO2 mitochondrial targeting sequence (N terminal on insert)

Resource Information

• Supplemental Documents:
  • pmxs-mito-gshf-full-sequence.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry