pEK20
(Plasmid #210582)

• Purpose: Reporter gene expression in B. dendrobatidis
• Depositing Lab: Lillian Fritz-Laylin
• Publication: Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;121(4):e2317928121. doi: 10.1073/pnas.2317928121. Epub 2024 Jan 18.

Backbone

• Vector backbone: pGI3EM17
• Backbone manufacturer: Edgar Medina
• Backbone size w/o insert (bp): 9378
• Total vector size (bp): 11422
• Vector type: Bacterial Expression, Yeast Expression, Luciferase
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BdGAPDHpro-Hshph-G4S-nanoluc-ScADH1ter
• Species: S. cerevisiae (budding yeast); Batrachochytrium dendrobatidis, Oplophorus gracilirostris
• Insert Size (bp): 2044
• Promoter: BdGAPDHpro
• Tag / Fusion Protein:
  • NanoLuc luciferase (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: gtaaaacgacggccagt
• 3′ sequencing primer: caggaaacagctatgac

Resource Information

• Supplemental Documents:
  • pek20_bdgapdhpro_hph-nanoluc.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.10.17.561934 for bioRxiv preprint.