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PKA-C-HiBiT
(Plasmid #211378)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211378 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone manufacturer
    Genscript
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6518
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human protein kinase cAMP-activated catalytic subunit alpha (PRKACA) transcript variant 1, with C-terminal HiBiT
  • Alt name
    PRKACA_C_HiBiT_C_His/Thrombin
  • Alt name
    PRKACA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1179
  • Mutation
    Kozak sequence for mammalian expression
  • GenBank ID
    BC108259.1
  • Entrez Gene
    PRKACA (a.k.a. CAFD1, PKACA, PPNAD4)
  • Promoter T7 Promotor
  • Tags / Fusion Proteins
    • HiBiT Tag (C terminal on insert)
    • 10x His-tag with thrombin site (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer T7 promotor primer #69348-3
  • 3′ sequencing primer T7 terminator primer #69337-3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    purchased as custom synthesis from Genscript

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PKA-C-HiBiT was a gift from James Inglese (Addgene plasmid # 211378 ; http://n2t.net/addgene:211378 ; RRID:Addgene_211378)

  • For your References section:

    A general assay platform to study protein pharmacology using ligand-dependent structural dynamics. Ciulla DA, Dranchak PK, Aitha M, van Neer RHP, Shah D, Tharakan R, Wilson KM, Wang Y, Braisted JC, Inglese J. Nat Commun. 2025 May 10;16(1):4342. doi: 10.1038/s41467-025-59658-6. 10.1038/s41467-025-59658-6 PubMed 40346061
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