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(Plasmid #211758)


Item Catalog # Description Quantity Price (USD)
Plasmid 211758 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    (Addgene #60958)
  • Modifications to backbone
    Replaced the human synapsin (hSyn) promotor with a ubiquitous CMV promotor to drive GFP expression
  • Vector type
    Mammalian Expression, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
    gRNA1 targeting VEGF-A
  • gRNA/shRNA sequence
  • Species
    H. sapiens (human), M. musculus (mouse); M. mulatta (rhesus macaque)
  • GenBank ID
  • Entrez Gene
    VEGFA (a.k.a. L-VEGF, MVCD1, VEGF, VPF)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SapI (unknown if destroyed)
  • 3′ cloning site SapI (unknown if destroyed)
  • 5′ sequencing primer GAGGGCCTATTTCCCATGAT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    px552-U6-gRNA1-CMV-eGFP was a gift from Glenn Yiu (Addgene plasmid # 211758 ; ; RRID:Addgene_211758)