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(Plasmid #212006)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212006 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 3962
  • Total vector size (bp) 8150
  • Modifications to backbone
    N-terminal EGFP removed
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    Tln1 (a.k.a. Tln)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry fluorescent protein (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer acgcaaatgggcggtagg
  • 3′ sequencing primer GATGAGTTTGGACAAACCAC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Talin1(ΔR4-12)-mCherry was a gift from Vesa Hytönen (Addgene plasmid # 212006 ; ; RRID:Addgene_212006)
  • For your References section:

    Talin-mediated force transmission and talin rod domain unfolding independently regulate adhesion signaling. Rahikainen R, Ohman T, Turkki P, Varjosalo M, Hytonen VP. J Cell Sci. 2019 Apr 3;132(7):jcs226514. doi: 10.1242/jcs.226514. 10.1242/jcs.226514 PubMed 30837291