pBbS8c_ddCpf1_final
(Plasmid
#212040)
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PurposeBacterial expression of the CRISPRi system for genome editing in E. coli
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 212040 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSC101
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameddCpf1
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SpeciesSynthetic
- Promoter L-arabinose-inducible promoter PBAD
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBbS8c_ddCpf1_final was a gift from Quanjiang Ji (Addgene plasmid # 212040 ; http://n2t.net/addgene:212040 ; RRID:Addgene_212040) -
For your References section:
BacPE: a versatile prime-editing platform in bacteria by inhibiting DNA exonucleases. Zhang H, Ma J, Wu Z, Chen X, Qian Y, Chen W, Wang Z, Zhang Y, Zhu H, Huang X, Ji Q. Nat Commun. 2024 Jan 27;15(1):825. doi: 10.1038/s41467-024-45114-4. 10.1038/s41467-024-45114-4 PubMed 38280845
