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PKC iota WT
(Plasmid #21252)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21252 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHACE
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKC iota
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1758
  • Entrez Gene
    PRKCI (a.k.a. DXS1179E, PKCI, nPKC-iota)
  • Tag / Fusion Protein
    • HA (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. T. Biden
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PKC iota WT was a gift from Bernard Weinstein (Addgene plasmid # 21252 ; http://n2t.net/addgene:21252 ; RRID:Addgene_21252)
  • For your References section:

    Roles of specific isoforms of protein kinase C in the transcriptional control of cyclin D1 and related genes. Soh JW, Weinstein IB. J Biol Chem. 2003 Sep 5. 278(36):34709-16. 10.1074/jbc.M302016200 PubMed 12794082