GfaABC1D-jGCaMP8m
(Plasmid
#213010)
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PurposeTo study calcium signaling in Astrocytes
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213010 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePZac2.1
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Backbone manufacturerKhakh lab
- Backbone size w/o insert (bp) 2821
- Total vector size (bp) 6378
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGfaABC1D-jGCaMP8m
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SpeciesM. musculus (mouse)
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Insert Size (bp)3557
- Promoter GfaABC1D
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer TCACTATAGGCTAGCCTCGAGCTCGGATCCACCACCATGCATCATCACCATCATCA
- 3′ sequencing primer AGGTTGATTATCGATAAGCGAATTGGATCCTTACTTCGCTGTCATCATTTG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byInsert subclone in PZAC2.1 vector from pGP-AAV-syn-jGCaMP8m-WPRE (#162375)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert subclone in PZAC2.1 vector from pGP-AAV-syn-jGCaMP8m-WPRE (#162375)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GfaABC1D-jGCaMP8m was a gift from Baljit Khakh (Addgene plasmid # 213010 ; http://n2t.net/addgene:213010 ; RRID:Addgene_213010) -
For your References section:
Crym-positive striatal astrocytes gate perseverative behaviour. Ollivier M, Soto JS, Linker KE, Moye SL, Jami-Alahmadi Y, Jones AE, Divakaruni AS, Kawaguchi R, Wohlschlegel JA, Khakh BS. Nature. 2024 Mar;627(8003):358-366. doi: 10.1038/s41586-024-07138-0. Epub 2024 Feb 28. 10.1038/s41586-024-07138-0 PubMed 38418885
Map uploaded by the depositor.
