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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21305 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepWZL Blast
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression, Retroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCCL5
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Alt namechemokine (C-C motif) ligand 5
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SpeciesH. sapiens (human)
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Entrez GeneCCL5 (a.k.a. D17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWZL Blasticidin human CCL5 was a gift from Bob Weinberg (Addgene plasmid # 21305) -
For your References section:
Mesenchymal stem cells within tumour stroma promote breast cancer metastasis. Karnoub AE, Dash AB, Vo AP, Sullivan A, Brooks MW, Bell GW, Richardson AL, Polyak K, Tubo R, Weinberg RA. Nature. 2007 Oct 4. 449(7162):557-63. 10.1038/nature06188 PubMed 17914389