Skip to main content
Addgene
Open Menu

ρ1-EM GABAA receptor in pEZT-BM
(Plasmid #213072)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213072 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 213072-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 213072-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pEZT-BM
  • Backbone manufacturer
    Addgene Plasmid #74099
  • Backbone size w/o insert (bp) 7436
  • Total vector size (bp) 9494
  • Vector type
    Mammalian Expression ; Bacmam, Baculovirus, oocyte expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human GABAa rho1 receptor
  • Alt name
    GABAa rho1
  • Alt name
    GABRR1
  • Alt name
    GABAC receptor
  • Species
    H. sapiens (human)
  • Mutation
    Encodes residues S58–L383 and D451–S479, separated by a sequence encoding the superfolder green fluorescent protein.81 Prior to the N-terminal truncation site, we added a membrane-translocation signal peptide from the human β3 GABAAR, a Twin-Strep tag, and a recognition site for tobacco etch virus for expression and purification purposes.
  • Entrez Gene
    GABRR1
  • Promoter CMV and T7
  • Tags / Fusion Proteins
    • superfolderGFP
    • Twin-Strep tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer WPRE Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Purchased from genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ρ1-EM GABAA receptor in pEZT-BM was a gift from Erik Lindahl (Addgene plasmid # 213072 ; http://n2t.net/addgene:213072 ; RRID:Addgene_213072)

  • For your References section:

    Structure and dynamics of differential ligand binding in the human rho-type GABA(A) receptor. Cowgill J, Fan C, Haloi N, Tobiasson V, Zhuang Y, Howard RJ, Lindahl E. Neuron. 2023 Nov 1;111(21):3450-3464.e5. doi: 10.1016/j.neuron.2023.08.006. Epub 2023 Sep 1. 10.1016/j.neuron.2023.08.006 PubMed 37659407
Related items: