SurA
(Plasmid
#213138)
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PurposeExpresses chaperone SurA
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213138 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsThe depositing lab uses BL21(DE3) for protein expression.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSurA (21-428)
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SpeciesE.coli
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Insert Size (bp)407
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Entrez GenesurA (a.k.a. b0053, ECK0054)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
6xHis-tag -- TEV cleavage site -- SurA
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SurA was a gift from Sebastian Hiller (Addgene plasmid # 213138 ; http://n2t.net/addgene:213138 ; RRID:Addgene_213138) -
For your References section:
High-throughput screening of BAM inhibitors in native membrane environment. Rath P, Hermann A, Schaefer R, Agustoni E, Vonach JM, Siegrist M, Miscenic C, Tschumi A, Roth D, Bieniossek C, Hiller S. Nat Commun. 2023 Sep 13;14(1):5648. doi: 10.1038/s41467-023-41445-w. 10.1038/s41467-023-41445-w PubMed 37704632
