pAAV-hSyn-DIO-myr-rsEGFP2- LDLR
(Plasmid
#213399)
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Purposedouble floxed, myristoylation site (myr) and LDLR, fused to reversibly switchable EGFP rsEGFP2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213399 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV
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Backbone manufacturerDeisseroth Lab
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namersEGFP2
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Alt namemyr-rsEGFP2-LDLR(Ct)
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GenBank IDAF425607
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Tags
/ Fusion Proteins
- myristoylation site (N terminal on insert)
- C-terminal (Ct) cytoplasmic domains of low density lipoprotein receptor (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn-DIO-myr-rsEGFP2- LDLR was a gift from Katrin Willig (Addgene plasmid # 213399 ; http://n2t.net/addgene:213399 ; RRID:Addgene_213399) -
For your References section:
Multi-label in vivo STED microscopy by parallelized switching of reversibly switchable fluorescent proteins. Willig KI, Wegner W, Muller A, Calvet-Fournier V, Steffens H. Cell Rep. 2021 Jun 1;35(9):109192. doi: 10.1016/j.celrep.2021.109192. 10.1016/j.celrep.2021.109192 PubMed 34077731