pcDNA3.1-VcnCS-E1015A
(Plasmid #213412)

• Purpose: Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).
• Depositing Lab: Brenton Hoffman
• Publication: Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi: 10.1038/s41467-023-43779-x.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5333
• Total vector size (bp): 9991
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: VcnCS-E1015A
• Alt name: VinCS-E1015A
• Alt name: VinculinCS-E1015A
• Species: G. gallus (chicken), Synthetic
• Insert Size (bp): 4658
• Mutation: Mutated vinculin glutamic acid 1015 to alanine (E1015A)
• Entrez Gene: VCL (a.k.a. VINC1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • pcdna3-1-vcncs-e1015a.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry