pTln1_spytag003_at 447aa
(Plasmid
#213641)
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PurposeExpresses Human Talin1 protein fused with SpyTag003 at the linker domain
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213641 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTalin 1
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SpeciesH. sapiens (human)
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Entrez GeneTLN1 (a.k.a. ILWEQ, TLN, talin-1)
- Promoter CMV
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Tags
/ Fusion Proteins
- SpyTag003 at the linker domain
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTln1_spytag003_at 447aa was a gift from Vesa Hytönen (Addgene plasmid # 213641 ; http://n2t.net/addgene:213641 ; RRID:Addgene_213641)