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M-PKD2 (OF2-3)
(Plasmid #21370)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21370 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 21370-D50 50 μg of DNA in Tris buffer $495
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DNA 21370-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCS2+MT
  • Backbone size w/o insert (bp) 4352
  • Vector type
    Mammalian Expression
  • Selectable markers
    none

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5a in dYT at 37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    autosomal dominant polycystic kidney disease type II
  • Alt name
    PKD2 wild-type
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3092
  • Mutation
    wild-type*
  • GenBank ID
    NM_000297
  • Entrez Gene
    PKD2 (a.k.a. APKD2, PC2, PKD4, Pc-2, TRPP2)
  • Tag / Fusion Protein
    • 6xMyc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site StuI (destroyed during cloning)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*Note that this construct has a rare SNP (rs1131408) at position 4:88967820 that results in a missense change (G449V). The polymorphism is not known to affect function.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M-PKD2 (OF2-3) was a gift from Gregory Germino (Addgene plasmid # 21370 ; http://n2t.net/addgene:21370 ; RRID:Addgene_21370)

  • For your References section:

    Co-assembly of polycystin-1 and -2 produces unique cation-permeable currents. Hanaoka K, Qian F, Boletta A, Bhunia AK, Piontek K, Tsiokas L, Sukhatme VP, Guggino WB, Germino GG. Nature. . 408(6815):990-4. 10.1038/35050128 PubMed 11140688
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