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(Plasmid #21367)


Item Catalog # Description Quantity Price (USD)
Plasmid 21367 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pCI (modified)
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4713
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    DH5a in dYT at 37oC
  • Copy number
    High Copy


  • Gene/Insert name
    autosomal dominant polycystic kidney disease type I
  • Alt name
    PKD1 wild-type
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    wild-type sequence*
  • GenBank ID
  • Entrez Gene
    PKD1 (a.k.a. PBP, PC1, Pc-1, TRPP1)
  • Tags / Fusion Proteins
    • EGFP (N terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*Please note: during Addgene's quality control process, the following mutations were found in PKD1: A691P, M792L, S999P, N1056T, T1724A, M1976V. The depositor has noted that the plasmid appears to be functional using several assays but it is not the standard PKD1 sequence in the database (Genbank ID L33243).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-WT (AF41) was a gift from Gregory Germino (Addgene plasmid # 21367 ; ; RRID:Addgene_21367)
  • For your References section:

    Cleavage of polycystin-1 requires the receptor for egg jelly domain and is disrupted by human autosomal-dominant polycystic kidney disease 1-associated mutations. Qian F, Boletta A, Bhunia AK, Xu H, Liu L, Ahrabi AK, Watnick TJ, Zhou F, Germino GG. Proc Natl Acad Sci U S A. 2002 Dec 24. 99(26):16981-6. 10.1073/pnas.252484899 PubMed 12482949