LbCas12a-niCPE
(Plasmid
#213747)
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PurposeFor circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213747 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMV-PE2
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Backbone manufacturerDavid Liu
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMCP-nLbCas12a-M-MLV RT∆RNase H
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SpeciesSynthetic; Lachnospiraceae bacterium
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MutationD156R, R1138A in LbCas12a
- Promoter CMV
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Tags
/ Fusion Proteins
- BPNLS (N terminal on insert)
- BPNLS (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LbCas12a-niCPE was a gift from Caixia Gao (Addgene plasmid # 213747 ; http://n2t.net/addgene:213747 ; RRID:Addgene_213747) -
For your References section:
Prime editing using CRISPR-Cas12a and circular RNAs in human cells. Liang R, He Z, Zhao KT, Zhu H, Hu J, Liu G, Gao Q, Liu M, Zhang R, Qiu JL, Gao C. Nat Biotechnol. 2024 Jan 10. doi: 10.1038/s41587-023-02095-x. 10.1038/s41587-023-02095-x PubMed 38200119
