pAAVS1-Neo-TNNT2-Puro
(Plasmid
#214016)
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PurposeExpression of cardiac troponin T
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Depositing Lab
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Depositing OrganizationNorthwestern University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214016 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 214016-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 214016-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepAAVS1-Nst-CAG-DEST (Addgene Plasmid #80489)
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Backbone manufacturerKnut Woltjen
- Backbone size w/o insert (bp) 3000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsIn mammalian cells, use Neomycin for selection of integrated constructs and use Puromycin for selection of cells with the TNNT2 promoter active
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTNNT2
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SpeciesH. sapiens (human)
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Entrez GeneTNNT2 (a.k.a. CMD1D, CMH2, CMPD2, LVNC6, RCM3, TnTC, cTnT)
Cloning Information
- Cloning method TOPO Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 214016-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 214016-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAVS1-Neo-TNNT2-Puro was a gift from Paul Burridge (Addgene plasmid # 214016 ; http://n2t.net/addgene:214016 ; RRID:Addgene_214016) -
For your References section:
Independent compartmentalization of functional, metabolic, and transcriptional maturation of hiPSC-derived cardiomyocytes. Fetterman KA, Blancard M, Lyra-Leite DM, Vanoye CG, Fonoudi H, Jouni M, DeKeyser JL, Lenny B, Sapkota Y, George AL Jr, Burridge PW. Cell Rep. 2024 May 28;43(5):114160. doi: 10.1016/j.celrep.2024.114160. Epub 2024 Apr 27. 10.1016/j.celrep.2024.114160 PubMed 38678564
