ART
(Plasmid
#214063)
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PurposeFluorescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR
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Depositing Lab
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Depositing OrganizationUniversity of Colorado Denver and Anschutz Medical Campus
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214063 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 214063-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 214063-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepRD_RIPE
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTurboRFP
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MutationLinker sequence present between RFP and GFP
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer N
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint.
DNA (Catalog # 214063-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 214063-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ART was a gift from Sujatha Jagannathan (Addgene plasmid # 214063 ; http://n2t.net/addgene:214063 ; RRID:Addgene_214063) -
For your References section:
A system of reporters for comparative investigation of EJC-independent and EJC-enhanced nonsense-mediated mRNA decay. Kolakada D, Campbell AE, Galvis LB, Li Z, Lore M, Jagannathan S. Nucleic Acids Res. 2024 Feb 20:gkae121. doi: 10.1093/nar/gkae121. 10.1093/nar/gkae121 PubMed 38375914
