pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7
(Plasmid
#214138)
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PurposeInsect cell expression of human 20S CP alpha-subunits
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214138 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACEBac1
- Total vector size (bp) 11905
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namePSMA1
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SpeciesH. sapiens (human)
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Entrez GenePSMA1 (a.k.a. HC2, HEL-S-275, NU, PROS30)
- Promoter polyhedrin
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namePSMA2
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SpeciesH. sapiens (human)
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Entrez GenePSMA2 (a.k.a. HC3, MU, PMSA2, PSC2)
- Promoter polyhedrin
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert namePSMA3
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SpeciesH. sapiens (human)
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Entrez GenePSMA3 (a.k.a. HC8, PSC3)
- Promoter polyhedrin
Cloning Information for Gene/Insert 3
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 4
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Gene/Insert namePSMA4
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SpeciesH. sapiens (human)
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Entrez GenePSMA4 (a.k.a. HC9, HsT17706, PSC9)
- Promoter polyhedrin
Cloning Information for Gene/Insert 4
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 5
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Gene/Insert namePSMA5
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SpeciesH. sapiens (human)
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Entrez GenePSMA5 (a.k.a. PSC5, ZETA)
- Promoter polyhedrin
Cloning Information for Gene/Insert 5
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 6
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Gene/Insert namePSMA6
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SpeciesH. sapiens (human)
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Entrez GenePSMA6 (a.k.a. IOTA, PROS27, p27K)
- Promoter polyhedrin
Cloning Information for Gene/Insert 6
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 7
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Gene/Insert namePSMA7
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SpeciesH. sapiens (human)
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Entrez GenePSMA7 (a.k.a. C6, HEL-S-276, HSPC, RC6-1, XAPC7)
- Promoter polyhedrin
Cloning Information for Gene/Insert 7
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note there is a deletion in Gentamycin that causes slightly reduced growth efficiency on (+Amp/Kan/Gen/Tet) compared to cells with a fully WT Gen resistance gene.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7 was a gift from Brenda Schulman (Addgene plasmid # 214138 ; http://n2t.net/addgene:214138 ; RRID:Addgene_214138) -
For your References section:
Visualizing chaperone-mediated multistep assembly of the human 20S proteasome. Adolf F, Du J, Goodall EA, Walsh RM Jr, Rawson S, von Gronau S, Harper JW, Hanna J, Schulman BA. Nat Struct Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9. 10.1038/s41594-024-01268-9 PubMed 38600324
Map uploaded by the depositor.
