delCMV-mCitrine
(Plasmid #214144)

• Purpose: Volume marker / Control sensor
• Depositing Lab: Leif Dehmelt
• Publication: Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi: 10.1038/s41467-023-43875-y.

Backbone

• Vector backbone: pmCitrine-N1
• Total vector size (bp): 4279
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCitrine
• Species: Aequorea victoria
• Insert Size (bp): 714
• Promoter: delCMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AseI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: whole plasmid sequencing

Resource Information

• Supplemental Documents:
  • delCMV-mCitrine.gb
• A portion of this plasmid was derived from a plasmid made by: Robert Campbell & Michael Davidson & Oliver Griesbeck & Roger Tsien (Addgene plasmid # 54594)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

the delCMV promotor was derived from Watanabe and Mitchison, 2002, Science, 295:1083