pAAV-Syn1-DIO-VGLUT2:miniSOG
(Plasmid
#214200)
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PurposeFor Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214200 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV DIO
- Total vector size (bp) 6962
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSlc17a6 tagged with miniSOG and hemagglutinin
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Alt nameVGLUT2
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Alt nameminiSOG
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Tag
/ Fusion Protein
- miniSOG (tagged with HA) is fused to the first intralumenal loop of rat Slc17a6 (VGLUT2)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-Syn1-DIO-VGLUT2:miniSOG was a gift from Thomas Hnasko (Addgene plasmid # 214200 ; http://n2t.net/addgene:214200 ; RRID:Addgene_214200) -
For your References section:
Genetic Probe for Visualizing Glutamatergic Synapses and Vesicles by 3D Electron Microscopy. Steinkellner T, Madany M, Haberl MG, Zell V, Li C, Hu J, Mackey M, Ramachandra R, Adams S, Ellisman MH, Hnasko TS, Boassa D. ACS Chem Neurosci. 2021 Feb 17;12(4):626-639. doi: 10.1021/acschemneuro.0c00643. Epub 2021 Feb 1. 10.1021/acschemneuro.0c00643 PubMed 33522227
