pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-mCherry
(Plasmid
#214471)
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PurposeLentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214471 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepHRsinUbEm
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameZFYVE27
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Alt nameSPG33
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Alt nameProtrudin
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SpeciesH. sapiens (human)
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Insert Size (bp)1251
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MutationS14D S25D S32D T261D
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Entrez GeneZFYVE27 (a.k.a. PROTRUDIN, SPG33)
Gene/Insert 2
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Gene/Insert namemCherry
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SpeciesA. marginale
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Insert Size (bp)711
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byVectorBuilder
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-mCherry was a gift from Richard Eva & Bart Nieuwenhuis (Addgene plasmid # 214471 ; http://n2t.net/addgene:214471 ; RRID:Addgene_214471)
