pGL4_EGR1p-BC0131-luc2
(Plasmid
#214647)
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Purposebarcoded assay, MAPK sensor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214647 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL4
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGR1 promoter with barcode 0131
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SpeciesSynthetic
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL4_EGR1p-BC0131-luc2 was a gift from Michael Wehr (Addgene plasmid # 214647 ; http://n2t.net/addgene:214647 ; RRID:Addgene_214647) -
For your References section:
Protocol for identifying properties of ERBB receptor antagonists using the barcoded ERBBprofiler assay. Popovic L, Rossner MJ, Wehr MC. STAR Protoc. 2024 Apr 16;5(2):102987. doi: 10.1016/j.xpro.2024.102987. 10.1016/j.xpro.2024.102987 PubMed 38635397