pMBP-HIS2-Vps20FL
(Plasmid #21490)

• Depositing Lab: James Hurley
• Publication: Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

Backbone

• Vector backbone: pHis2
• Backbone size w/o insert (bp): 5549
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VPS20
• Alt name: CHM6
• Alt name: VPT20
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 663
• Mutation: none
• Entrez Gene: VPS20 (a.k.a. YMR077C, CHM6, VPL10, VPT20)
• Tags / Fusion Proteins:
  • 6x His (N terminal on backbone)
  • MBP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: N/A (RF cloning used) (not destroyed)
• 5′ sequencing primer: T7 promotor

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry