Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pKH152_PB_flag-LC3B_LDSmut_IRES-BFP
(Plasmid #214977)

Print

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 214977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    piggybac
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLAG-LC3B_mut
  • Species
    H. sapiens (human)
  • Mutation
    LDS mutant
  • Entrez Gene
    MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKH152_PB_flag-LC3B_LDSmut_IRES-BFP was a gift from Wade Harper (Addgene plasmid # 214977 ; http://n2t.net/addgene:214977 ; RRID:Addgene_214977)

  • For your References section:

    Proteome census upon nutrient stress reveals Golgiphagy membrane receptors. Hickey KL, Swarup S, Smith IR, Paoli JC, Miguel Whelan E, Paulo JA, Harper JW. Nature. 2023 Nov;623(7985):167-174. doi: 10.1038/s41586-023-06657-6. Epub 2023 Sep 27. 10.1038/s41586-023-06657-6 PubMed 37757899
Related items: