pYZ1389
(Plasmid
#215348)
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PurposeNla CRISPRi Delivery Vector
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Depositing Lab
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Depositing OrganizationNorthwestern University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 215348 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 215348-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 215348-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGCC4
- Backbone size w/o insert (bp) 9268
- Total vector size (bp) 12960
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Vector typeCRISPR ; Nla i Delivery Vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsThe plasmid can be maintained using either Kanamycin or Erythromycin separately.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNla CRISPRi Genes (Cas5, Cas8c, Cas7)
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SpeciesNeisseria lactamica
Resource Information
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A portion of this plasmid was derived from a plasmid made byYan Zhang -- University of Michigan
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 215348-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 215348-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYZ1389 was a gift from Hank Seifert (Addgene plasmid # 215348 ; http://n2t.net/addgene:215348 ; RRID:Addgene_215348) -
For your References section:
Development and implementation of a Type I-C CRISPR-based programmable repression system for Neisseria gonorrhoeae. Geslewitz WE, Cardenas A, Zhou X, Zhang Y, Criss AK, Seifert HS. mBio. 2023 Dec 21:e0302523. doi: 10.1128/mbio.03025-23. 10.1128/mbio.03025-23 PubMed 38126782
Map uploaded by the depositor.
