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Actin Binding Domain Tension Sensor
(Plasmid #215368)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215368 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 215368-D50 50 μg of DNA in Tris buffer $495
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DNA 215368-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5397
  • Total vector size (bp) 7263
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Actin Binding Domain Tension Sensor
  • Alt name
    ADBTS
  • Species
    Synthetic
  • Insert Size (bp)
    1866
  • Promoter CMV

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dyche Mullins (F-tractin and Linker 1 from Addgene plasmid # 58473) and Martin Schwartz (Original TSMod from Addgene plasmid # 26021).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

F-tractin is based on the rat neuronal inositol 1,4,5-triphosphate 3-kinase A (ITPKA) actin-binding domain.

Please visit https://www.biorxiv.org/content/10.1101/2024.01.10.575065v1 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Actin Binding Domain Tension Sensor was a gift from Brenton Hoffman (Addgene plasmid # 215368 ; http://n2t.net/addgene:215368 ; RRID:Addgene_215368)

  • For your References section:

    Detection of fluorescent protein mechanical switching in cellulo. Shoyer TC, Collins KL, Ham TR, Blanchard AT, Malavade JN, Johns BA, West JL, Hoffman BD. Cell Rep Methods. 2024 Jul 15;4(7):100815. doi: 10.1016/j.crmeth.2024.100815. Epub 2024 Jul 9. 10.1016/j.crmeth.2024.100815 PubMed 38986612
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