NfPolyA_pET21b
(Plasmid #215413)

• Purpose: Expression construct for NfPolyA gene, codon optimized for expression in E. coli
• Depositing Lab: Gregg Beckham
• Publication: Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi: 10.1038/s41467-024-45523-5.

Backbone

• Vector backbone: pET21b
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NfPolyA
• Alt name: Amidase from Nocardia farcinica
• Species: Nocardia farcinica

Cloning Information

• Cloning method: Gene Synthesis

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene from amidase described in Guo, Y., Chen, S., Su, L., Wu, J. & Chen, J. Cloning, expression, and characterization of polyamidase from Nocardia farcinica and its application to polyamide modification. Biotechnol. Bioprocess Eng. 18, 1067-1075 (2013).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry