NylCp2-TS_pET28a
(Plasmid #215421)

• Purpose: Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli
• Depositing Lab: Gregg Beckham
• Publication: Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi: 10.1038/s41467-024-45523-5.

Backbone

• Vector backbone: pET28a
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NylCp2-TS
• Alt name: Nylon oligomer hydrolase from Kocuria sp.
• Species: Kocuria sp.
• Mutation: D122G/H130Y/D36A/E263Q

Cloning Information

• Cloning method: Gene Synthesis

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene from nylon oligomer hydrolase described in Negoro, S. et al. Structural basis of the correct subunit assembly, aggregation, and intracellular degradation of nylon hydrolase. Sci. Rep. 8, 9725 (2018).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry