CAGGS-losSTOPlox-ClaI flp-in Notes
Addgene diagnostic digest
This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid # 21548 with the enzymes NheI-HF and SacI.
We expect the single digest to give 1 band at 8.3kb and the double digest to give 2 bands at 7.3 and 1kb.
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid CAGGS-losSTOPlox-ClaI flp-in with the following enzymes: NheI-HF and ClaI.
We expect one band at 8.3kb. This plasmid is not giving the expected pattern.