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pMT2-HA-MKK3
(Plasmid #21580)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21580 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMT2
  • Backbone manufacturer
    Unknown
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitogen-activated protein kinase kinase 3
  • Alt name
    MKK3
  • Species
    H. sapiens (human)
  • Entrez Gene
    MAP2K3 (a.k.a. MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is an amino acid change from A to T at position 100, as seen from Addgene sequencing. Author does not feel this will cause any problems, and is not near critical residues.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT2-HA-MKK3 was a gift from John Kyriakis (Addgene plasmid # 21580 ; http://n2t.net/addgene:21580 ; RRID:Addgene_21580)
  • For your References section:

    Cdc42Hs, but not Rac1, inhibits serum-stimulated cell cycle progression at G1/S through a mechanism requiring p38/RK. Molnar A, Theodoras AM, Zon LI, Kyriakis JM. J Biol Chem. 1997 May 16. 272(20):13229-35. 10.1074/jbc.272.20.13229 PubMed 9148940