Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21633||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerDr. David Russell, UTSW
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameMig6, Ralt, ERRFI
SpeciesR. norvegicus (rat)
Insert Size (bp)1380
Entrez GeneErrfi1 (a.k.a. RGD1307599, Ralt, Xxx, gene 33)
/ Fusion Protein
- FLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Eco RI (not destroyed)
- 3′ cloning site Sal I (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Terms and Licenses
Plasmid was also used in Xu et al. 2005
the construct can be used in cardiomyocytes
There is a V to L amino acid change at position 6. The lab feels it is conservative enough not to matter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV5-FLAG-Gene33 was a gift from John Kyriakis (Addgene plasmid # 21633 ; http://n2t.net/addgene:21633 ; RRID:Addgene_21633)
For your References section:Gene 33/Mig-6, a transcriptionally inducible adapter protein that binds GTP-Cdc42 and activates SAPK/JNK. A potential marker transcript for chronic pathologic conditions, such as diabetic nephropathy. Possible role in the response to persistent stress. Makkinje A, Quinn DA, Chen A, Cadilla CL, Force T, Bonventre JV, Kyriakis JM. J Biol Chem. 2000 Jun 9. 275(23):17838-47. 10.1074/jbc.M909735199 PubMed 10749885