Mito-FAST-IRES-iRFP670
(Plasmid
#216346)
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PurposeFor testing localization of pFAST and iRFP670 proteins to mitochondria
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216346 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneAddgene #70113
- Backbone size w/o insert (bp) 3767
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCox8_pFAST-IRES-Tomm20_iRFP670
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SpeciespFAST derived from photoactive yellow protein (PYP) from H. Halophila; iRPF670 derived from Rhodopseudomonas palustris CGA009
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Insert Size (bp)2584
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Mutationn/a
- Promoter CMV
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Tag
/ Fusion Protein
- myc for FAST and iRF670 proteins (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
SV40 promoter partial
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mito-FAST-IRES-iRFP670 was a gift from Alison Tebo (Addgene plasmid # 216346 ; http://n2t.net/addgene:216346 ; RRID:Addgene_216346)
