FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1
(Plasmid
#216378)
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PurposeBaculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216378 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFastBac1
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Backbone manufacturerGibco
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Vector typeInsect Expression ; Baculovirus
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFrizzled-4
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Alt nameFzd4
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SpeciesM. musculus (mouse)
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Insert Size (bp)1600
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Entrez GeneFzd4 (a.k.a. Fz4)
- Promoter Polyhedrin
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Tag
/ Fusion Protein
- HA signal sequence-FLAG (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1 was a gift from William Weis (Addgene plasmid # 216378 ; http://n2t.net/addgene:216378 ; RRID:Addgene_216378) -
For your References section:
PI(4,5)P(2)-stimulated positive feedback drives the recruitment of Dishevelled to Frizzled in Wnt-beta-catenin signaling. Mahoney JP, Bruguera ES, Vasishtha M, Killingsworth LB, Kyaw S, Weis WI. Sci Signal. 2022 Aug 23;15(748):eabo2820. doi: 10.1126/scisignal.abo2820. Epub 2022 Aug 23. 10.1126/scisignal.abo2820 PubMed 35998232
