pPOM019_P2_pRPL2102
(Plasmid
#216444)
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PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216444 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepYTK001
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Vector typeYeast Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePromoter rpl2102
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SpeciesS. pombe (fission yeast)
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Insert Size (bp)1023
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MutationRemoval of any BsaI // BsmBI restriction site
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPOM019_P2_pRPL2102 was a gift from Tomáš Pluskal (Addgene plasmid # 216444 ; http://n2t.net/addgene:216444 ; RRID:Addgene_216444) -
For your References section:
POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic Biology. Hebra T, Smrckova H, Elkatmis B, Prevorovsky M, Pluskal T. ACS Synth Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529. 10.1021/acssynbio.3c00529 PubMed 37991801
