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pET3C-ARL16(CO)-His6
(Plasmid #216485)

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  • Purpose
    Used for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.
  • Depositing Lab
    Richard Kahn
  • Depositing Organization
    Emory University
    Located in United States of America
  • Publication
    pcDNA3.1-HsARL16-His6 (unpublished) ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216485 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET3C
  • Backbone size w/o insert (bp) 4638
  • Total vector size (bp) 5196
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ADP-ribosylation factor-like 16
  • Alt name
    ARL16
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    558
  • GenBank ID
    NM_001040025.3 339231
  • Entrez Gene
    ARL16
  • Promoter T7
  • Tag / Fusion Protein
    • includes GSGSGSHHHHHH (His6) tag at C-terminus (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET3C-ARL16(CO)-His6 was a gift from Richard Kahn (Addgene plasmid # 216485 ; http://n2t.net/addgene:216485 ; RRID:Addgene_216485)
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