T2(2)-ori
(Plasmid
#216627)
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Purpose(Empty Backbone) Knockout vector for Bacillus velenzensis
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216627 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneT2(2)-ori
- Backbone size (bp) 4294
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Modifications to backboneaddition of golden gate sites
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Golden Gate
Resource Information
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A portion of this plasmid was derived from a plasmid made byGaofu Qi
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
T2(2)-ori was a gift from Adam Arkin (Addgene plasmid # 216627 ; http://n2t.net/addgene:216627 ; RRID:Addgene_216627)