MO70 Orai1 N223A
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21663||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7480
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesH. sapiens (human)
Entrez GeneORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)
/ Fusion Protein
- Flag (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
See author's map for image of empty MO70 vector (MO70 contains IRES-GFP after the multiple cloning site).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MO70 Orai1 N223A was a gift from Anjana Rao (Addgene plasmid # 21663 ; http://n2t.net/addgene:21663 ; RRID:Addgene_21663)
For your References section:Biochemical and functional characterization of Orai proteins. Gwack Y, Srikanth S, Feske S, Cruz-Guilloty F, Oh-hora M, Neems DS, Hogan PG, Rao A. J Biol Chem. 2007 Jun 1. 282(22):16232-43. 10.1074/jbc.M609630200 PubMed 17293345
Map uploaded by the depositor.