pNTI822
(Plasmid
#216631)
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PurposeTet-inducible cpTurboID (V5) for Sleeping Beauty transposon system
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216631 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSBtet-Hyg
- Backbone size w/o insert (bp) 6011
- Total vector size (bp) 7070
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Vector typeMammalian Expression ; Sleeping Beauty Transposon System
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecpTurboID
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SpeciesSynthetic
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Insert Size (bp)1059
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MutationL120P
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNTI822 was a gift from Nicholas Ingolia (Addgene plasmid # 216631 ; http://n2t.net/addgene:216631 ; RRID:Addgene_216631) -
For your References section:
Molecular recording of calcium signals via calcium-dependent proximity labeling. Kim J.W., Yong A.J.H., Aisenberg E.E. et al.. Nat Chem Biol (2024) 10.1038/s41589-024-01603-7
