FP5369 KIF17MD-flag- FIREmate
(Plasmid
#216724)
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PurposeExpresses motor domain of KIF17 fused to FIREmate in mamallian cells
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Depositing Lab
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Depositing OrganizationInstitut Curie
Located in France -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216724 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 216724-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 216724-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-N3
- Total vector size (bp) 5998
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKIF17
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SpeciesH. sapiens (human)
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Insert Size (bp)2028
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Mutation1-547
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GenBank ID57576
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Entrez GeneKIF17 (a.k.a. KIF17B, KIF3X, KLP-2, OSM-3)
- Promoter CMV
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Tags
/ Fusion Proteins
- Flag (C terminal on insert)
- FIREmate (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pEGFP-FP
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 216724-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 216724-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FP5369 KIF17MD-flag- FIREmate was a gift from Franck Perez (Addgene plasmid # 216724 ; http://n2t.net/addgene:216724 ; RRID:Addgene_216724) -
For your References section:
A fluorogenic chemically induced dimerization technology for controlling, imaging and sensing protein proximity. Bottone S, Joliot O, Cakil ZV, El Hajji L, Rakotoarison LM, Boncompain G, Perez F, Gautier A. Nat Methods. 2023 Aug 28. doi: 10.1038/s41592-023-01988-8. 10.1038/s41592-023-01988-8 PubMed 37640938
Map uploaded by the depositor.
