LifeAct-MTED
(Plasmid
#217498)
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PurposeExpresses LifeAct-mScarlet-MTED for targeting fluorescent, MT depolymerizing peptide MTED to f-actin
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Depositing Lab
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Depositing OrganizationUniversity of Wisconsin, Madison
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 217498 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 217498-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 217498-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneTet-On
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Backbone manufacturerDavid Root, RRID: Addgene_41393
- Backbone size w/o insert (bp) 4621
- Total vector size (bp) 5461
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLifeAct-mScarlet-MTED
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Alt nameLifeAct-MTED
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Alt nameMicrotubule Elimination Domain of Efa6 protein
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SpeciesD. melanogaster (fly)
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Insert Size (bp)840
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 217498-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 217498-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LifeAct-MTED was a gift from Erik Dent (Addgene plasmid # 217498 ; http://n2t.net/addgene:217498 ; RRID:Addgene_217498) -
For your References section:
A methodology for specific disruption of microtubule polymerization into dendritic spines. Holland ED, Miller HL, Millette MM, Taylor RJ, Drucker GL, Dent EW. Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi: 10.1091/mbc.E24-02-0093. Epub 2024 Apr 17. 10.1091/mbc.E24-02-0093 PubMed 38630519
Map uploaded by the depositor.
