Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21761||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5200
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameECFP -EYFP linked by flexible linker containing two SGGSGG repeats
Insert Size (bp)1670
/ Fusion Protein
- His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byECFP and EYFP genes were obtained from BD Biosciences
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28CLY2 was a gift from Maarten Merkx (Addgene plasmid # 21761 ; http://n2t.net/addgene:21761 ; RRID:Addgene_21761)
For your References section:Quantitative understanding of the energy transfer between fluorescent proteins connected via flexible peptide linkers. Evers TH, van Dongen EM, Faesen AC, Meijer EW, Merkx M. Biochemistry. 2006 Nov 7. 45(44):13183-92. 10.1021/bi061288t PubMed 17073440