PBac-15XQUAS-unc84:2XeGFP-3XP3-ECFP
(Plasmid
#217614)
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PurposeLabels the inner nuclear membrane of A. aegypti neurons with GFP
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 217614 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonePBac
- Backbone size w/o insert (bp) 3999
- Total vector size (bp) 11369
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Vector typePBac
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name15XQUAS-unc84:2XeGFP-3xP3-ECFP
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Alt nameQUAS-unc84:GFP
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SpeciesC. elegans (nematode)
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Insert Size (bp)7309
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GenBank ID181480
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Entrez Geneunc-84 (a.k.a. CELE_F54B11.3)
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Tag
/ Fusion Protein
- eGFP
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 forward
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byUnc-84 originally cloned from pMUH_unc84_2XGFP (Plasmid #46023) by Gilbert L. Henry.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.08.21.608847 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PBac-15XQUAS-unc84:2XeGFP-3XP3-ECFP was a gift from Carolyn McBride (Addgene plasmid # 217614 ; http://n2t.net/addgene:217614 ; RRID:Addgene_217614) -
For your References section:
Odorant receptor coexpression and multi-expression in the dengue mosquito. David D, dos Anjos VL, Kotb SM, Edwards M, Metz HC, Tian D, Zhao Z, Zung JL, Rose NH, McBride CS. Nat Commun (2026). https://doi.org/10.1038/s41467-026-76682-2 10.1038/s41467-026-76682-2