Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

HA-MBP-TEVpCS-ADGRE5 CTF-FLAG
(Plasmid #217693)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217693 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFuse-hIgG1-Fc2
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ADGRE5
  • Alt name
    CD97
  • Species
    H. sapiens (human)
  • Mutation
    Deletion of residues 1-530
  • Entrez Gene
    ADGRE5 (a.k.a. CD97, TM7LN1)
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-MBP-TEVpCS-ADGRE5 CTF-FLAG was a gift from Stephen Blacklow (Addgene plasmid # 217693 ; http://n2t.net/addgene:217693 ; RRID:Addgene_217693)
  • For your References section:

    Heterogeneity of Tethered Agonist Signaling in Adhesion G Protein-Coupled Receptors. Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse AC, Blacklow SC. bioRxiv 2022.07.14.500097 10.1101/2022.07.14.500097